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KMID : 0613820100200030411
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Journal of Life Science
2010 Volume.20 No. 3 p.411 ~ p.415
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Construction of Web-Based Database for Anisakis Research
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Lee Yong-Seok
Baek Moon-Ki
Jo Yong-Hun
Kang Se-Won
Lee Jae-Bong
Han Yeon-Soo
Cha Hee-Jae
Yu Hak-Sun
Ock Mee-Sun
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Abstract
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Anisakis simplex is one of the parasitic nematodes, and has a complex life cycle in crustaceans, fish, squid or whale. When people eat under-processed or raw fish, it causes anisakidosis and also plays a critical role in inducing serious allergic reactions in humans. However, no web-based database on A. simplex at the level of DNA or protein has been so far reported. In this context, we constructed a web-based database for Anisakis research. To build up the web-based database for Anisakis research, we proceeded with the following measures: First, sequences of order Ascaridida were downloaded and translated into the multifasta format which was stored as database for stand-alone BLAST. Second, all of the nucleotide and EST sequences were clustered and assembled. And EST sequences were translated into amino acid sequences for Nuclear Localization Signal prediction. In addition, we added the vector, E. coli, and repeat sequences into the database to confirm a potential contamination. The web-based database gave us several advantages. Only data that agrees with the nucleotide sequences directly related with the order Ascaridida can be found and retrieved when searching BLAST. It is also very convenient to confirm contamination when making the cDNA or genomic library from Anisakis. Furthermore, BLAST results on the Anisakis sequence information can be quickly accessed. Taken together, the Web-based database on A. simplex will be valuable in developing species specific PCR markers and in studying SNP in A. simplex-related researches in the future.
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KEYWORD
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Web-based database, Anisakis, interface, sequence information
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FullTexts / Linksout information
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Listed journal information
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